{"id":9059802677407,"title":"Colifast Field Kit","handle":"colifast-field-kit","description":"\u003cbody\u003e\n\n \u003ch1\u003eColifast Field Kit\u003c\/h1\u003e\n\n \u003cp\u003eColifast Field Kit is a portable tool for field detection of coliform bacteria in water. Three methods are available with the kit:\u003c\/p\u003e\n\n \u003cul\u003e\n \u003cli\u003eThe Rapid method\u003c\/li\u003e\n \u003cli\u003ePresence\/absence\u003c\/li\u003e\n \u003cli\u003eQuantitative (MPN)\u003c\/li\u003e\n \u003c\/ul\u003e\n\n \u003cp\u003eThe main method of the kit is a rapid method that provides an answer as early as 75 minutes, 2 hours at the latest (limited to levels above 500 cfu\/100ml). In addition, the kit includes methods for a quantitative MPN (Most Probable Number) method and presence\/absence results. The procedure is very easy to perform, and requires no laboratory skills. Colifast Field Kit is delivered in a robust and portable metal case, and all equipment required for the water analysis is included. The Colifast Micro Detector (CMD) is used in combination with a growth medium for specific detection of target bacteria. Combined, they provide a simple analysis and rapid results that quantify the amount of coliform and thermotolerant bacteria in water. The method is based upon the patented technology of Colifast AS.\u003c\/p\u003e\n\n \u003ch2\u003eSpreadsheet for calculating bacterial concentrations when conducting the Rapid method.\u003c\/h2\u003e\n\n \u003cp\u003eWebTool for calculating bacterial concentrations when using the rapid method\u003c\/p\u003e\n\n \u003ch2\u003eMethod\u003c\/h2\u003e\n\n \u003cp\u003eThe Colifast technology is based on a chemical reaction between a substrate in the growth medium and enzymes produced by the coliform bacteria. The bacterial enzyme β-D-galactosidase hydrolyses the substrate 4-methylumbelliferyl-β-D-galactoside, which results in the release of the fluorescent product 4-methylumbelliferone (MU). In addition to substrate, activators and growth factors, the medium comprises inhibitors that prevent the growth of non-coliform bacteria. The results are measured in fluorescent units. An increase in the number of bacteria corresponds to an increased amount of β-D-galactosidase (enzyme). The subsequent increase in the amount of MU (fluorescent product) results in a higher value on the instrument (Colifast Micro Detector). The incubation temperature selects for thermotolerant coliforms at 44 °C and total coliforms at 37 °C.\u003c\/p\u003e\n\n \u003ch2\u003eColifast Field kit Content\u003c\/h2\u003e\n\n \u003cul\u003e\n \u003cli\u003eColifast Micro Detector with storage pouch\u003c\/li\u003e\n \u003cli\u003eIncubator with thermometer, 220 V\u003c\/li\u003e\n \u003cli\u003eCar converter 12 V – 220 V\u003c\/li\u003e\n \u003cli\u003eCable for battery poles\u003c\/li\u003e\n \u003cli\u003e18 Prefilled vials with Colifast 6 medium\u003c\/li\u003e\n \u003cli\u003e2 dropper bottles with 0.5 M NaOH solution (developer)\u003c\/li\u003e\n \u003cli\u003eVial with Blank (calibration)\u003c\/li\u003e\n \u003cli\u003eVial with Cal 48 (calibration)\u003c\/li\u003e\n \u003cli\u003eDropper bottle with 10% Na-thiosulfate solution (chloride neutralization)\u003c\/li\u003e\n \u003cli\u003e100 cuvettes (disposable)\u003c\/li\u003e\n \u003cli\u003e100 cuvette caps (disposable)\u003c\/li\u003e\n \u003cli\u003eCuvette rack\u003c\/li\u003e\n \u003cli\u003e20 large pastettes for sub-sampling (3 ml graded, one for each test)\u003c\/li\u003e\n \u003cli\u003e3 sterile 10 ml syringes for the addition of the water sample (can be reused after wash)\u003c\/li\u003e\n \u003cli\u003eEthanol tissues (5 pcs) for disinfection\u003c\/li\u003e\n \u003cli\u003e2 sterile filters. For control samples, fits on the syringe\u003c\/li\u003e\n \u003cli\u003eManual\u003c\/li\u003e\n \u003cli\u003eQuick guide\u003c\/li\u003e\n \u003c\/ul\u003e\n\n\u003c\/body\u003e","published_at":"2023-10-03T21:36:34+02:00","created_at":"2023-10-03T21:36:34+02:00","vendor":"SelectTechWesbite","type":"Industrial","tags":["Colifast","Colifast Field Kit","coliform test","E.coli","fecal testing","Field test kit","Industrial","most probable number test","presence\/ absence test","rapid screening test"],"price":0,"price_min":0,"price_max":0,"available":true,"price_varies":false,"compare_at_price":null,"compare_at_price_min":0,"compare_at_price_max":0,"compare_at_price_varies":false,"variants":[{"id":44522725474463,"title":"Default Title","option1":"Default Title","option2":null,"option3":null,"sku":null,"requires_shipping":true,"taxable":true,"featured_image":null,"available":true,"name":"Colifast Field Kit","public_title":null,"options":["Default Title"],"price":0,"weight":0,"compare_at_price":null,"inventory_management":"shopify","barcode":null,"requires_selling_plan":false,"selling_plan_allocations":[]}],"images":["\/\/selectech.co.za\/cdn\/shop\/files\/colifast_selectech-co-za-Shop-at-Selectech_for--lab-equipment.jpg?v=1696362394","\/\/selectech.co.za\/cdn\/shop\/files\/colifast.jpg?v=1696362510"],"featured_image":"\/\/selectech.co.za\/cdn\/shop\/files\/colifast_selectech-co-za-Shop-at-Selectech_for--lab-equipment.jpg?v=1696362394","options":["Title"],"media":[{"alt":null,"id":29572026794143,"position":1,"preview_image":{"aspect_ratio":1.0,"height":1024,"width":1024,"src":"\/\/selectech.co.za\/cdn\/shop\/files\/colifast_selectech-co-za-Shop-at-Selectech_for--lab-equipment.jpg?v=1696362394"},"aspect_ratio":1.0,"height":1024,"media_type":"image","src":"\/\/selectech.co.za\/cdn\/shop\/files\/colifast_selectech-co-za-Shop-at-Selectech_for--lab-equipment.jpg?v=1696362394","width":1024},{"alt":null,"id":29572030464159,"position":2,"preview_image":{"aspect_ratio":1.0,"height":500,"width":500,"src":"\/\/selectech.co.za\/cdn\/shop\/files\/colifast.jpg?v=1696362510"},"aspect_ratio":1.0,"height":500,"media_type":"image","src":"\/\/selectech.co.za\/cdn\/shop\/files\/colifast.jpg?v=1696362510","width":500}],"requires_selling_plan":false,"selling_plan_groups":[],"content":"\u003cbody\u003e\n\n \u003ch1\u003eColifast Field Kit\u003c\/h1\u003e\n\n \u003cp\u003eColifast Field Kit is a portable tool for field detection of coliform bacteria in water. Three methods are available with the kit:\u003c\/p\u003e\n\n \u003cul\u003e\n \u003cli\u003eThe Rapid method\u003c\/li\u003e\n \u003cli\u003ePresence\/absence\u003c\/li\u003e\n \u003cli\u003eQuantitative (MPN)\u003c\/li\u003e\n \u003c\/ul\u003e\n\n \u003cp\u003eThe main method of the kit is a rapid method that provides an answer as early as 75 minutes, 2 hours at the latest (limited to levels above 500 cfu\/100ml). In addition, the kit includes methods for a quantitative MPN (Most Probable Number) method and presence\/absence results. The procedure is very easy to perform, and requires no laboratory skills. Colifast Field Kit is delivered in a robust and portable metal case, and all equipment required for the water analysis is included. The Colifast Micro Detector (CMD) is used in combination with a growth medium for specific detection of target bacteria. Combined, they provide a simple analysis and rapid results that quantify the amount of coliform and thermotolerant bacteria in water. The method is based upon the patented technology of Colifast AS.\u003c\/p\u003e\n\n \u003ch2\u003eSpreadsheet for calculating bacterial concentrations when conducting the Rapid method.\u003c\/h2\u003e\n\n \u003cp\u003eWebTool for calculating bacterial concentrations when using the rapid method\u003c\/p\u003e\n\n \u003ch2\u003eMethod\u003c\/h2\u003e\n\n \u003cp\u003eThe Colifast technology is based on a chemical reaction between a substrate in the growth medium and enzymes produced by the coliform bacteria. The bacterial enzyme β-D-galactosidase hydrolyses the substrate 4-methylumbelliferyl-β-D-galactoside, which results in the release of the fluorescent product 4-methylumbelliferone (MU). In addition to substrate, activators and growth factors, the medium comprises inhibitors that prevent the growth of non-coliform bacteria. The results are measured in fluorescent units. An increase in the number of bacteria corresponds to an increased amount of β-D-galactosidase (enzyme). The subsequent increase in the amount of MU (fluorescent product) results in a higher value on the instrument (Colifast Micro Detector). The incubation temperature selects for thermotolerant coliforms at 44 °C and total coliforms at 37 °C.\u003c\/p\u003e\n\n \u003ch2\u003eColifast Field kit Content\u003c\/h2\u003e\n\n \u003cul\u003e\n \u003cli\u003eColifast Micro Detector with storage pouch\u003c\/li\u003e\n \u003cli\u003eIncubator with thermometer, 220 V\u003c\/li\u003e\n \u003cli\u003eCar converter 12 V – 220 V\u003c\/li\u003e\n \u003cli\u003eCable for battery poles\u003c\/li\u003e\n \u003cli\u003e18 Prefilled vials with Colifast 6 medium\u003c\/li\u003e\n \u003cli\u003e2 dropper bottles with 0.5 M NaOH solution (developer)\u003c\/li\u003e\n \u003cli\u003eVial with Blank (calibration)\u003c\/li\u003e\n \u003cli\u003eVial with Cal 48 (calibration)\u003c\/li\u003e\n \u003cli\u003eDropper bottle with 10% Na-thiosulfate solution (chloride neutralization)\u003c\/li\u003e\n \u003cli\u003e100 cuvettes (disposable)\u003c\/li\u003e\n \u003cli\u003e100 cuvette caps (disposable)\u003c\/li\u003e\n \u003cli\u003eCuvette rack\u003c\/li\u003e\n \u003cli\u003e20 large pastettes for sub-sampling (3 ml graded, one for each test)\u003c\/li\u003e\n \u003cli\u003e3 sterile 10 ml syringes for the addition of the water sample (can be reused after wash)\u003c\/li\u003e\n \u003cli\u003eEthanol tissues (5 pcs) for disinfection\u003c\/li\u003e\n \u003cli\u003e2 sterile filters. For control samples, fits on the syringe\u003c\/li\u003e\n \u003cli\u003eManual\u003c\/li\u003e\n \u003cli\u003eQuick guide\u003c\/li\u003e\n \u003c\/ul\u003e\n\n\u003c\/body\u003e"}